General Cell Based Assays
Meso Scale Discovery U-PLEX Mouse IL-15 ASY 25 PL
The Meso Scale Discovery U-PLEX Mouse IL-15 Assay Kit is an electrochemiluminescent immunoassay for the quantitative measurement of mouse interleukin 15 (IL-15) in serum plasma and cell culture supernatants The assay uses MSD s proprietary SULFO-TAG labels and multi-array technology to provide sensitive and reproducible quantitation of mouse IL-15 with a lower limit of detection of 1 3 pg/mL and an upper limit of quantification of 10 000 pg/mL The kit includes a 96-well plate coated with capture antibody detection antibody conjugated to MSD SULFO-TAG calibrators and sample diluent The assay takes approximately 3 5 hours to complete after a 2 hour incubation
Eurofins Discoverx PathHunter eXpress C5AR1 CHO-K1 β-Arrestin GPCR Assay, 10-Plate Kit. (93-0557E2CP0L)
The PathHunter C5AR1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. C5a) based activation of C5AR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged C5AR1 and EA-tagged β-Arrestin-2. Activation of C5AR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress HTR2C (VNV) U2OS β-Arrestin GPCR Assay, 2pl Kit. (93-0938E3CP19M)
The PathHunter HTR2C(VNV) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. serotonin) based activation of HTR2C(VNV) GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged HTR2C(VNV) and EA-tagged β-Arrestin-2. Activation of HTR2C(VNV) stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Aat Bioquest Cal-590™ AM, 10x50µg
Cal-590™ has been developed to improve Rhod-2 cell loading and calcium response while maintaining the spectral wavelength of Rhod-2, making it compatible with TRITC/Cy3 filter set. In CHO and HEK cells Cal-590™ AM has cellular calcium response that is much more sensitive than Rhod-2 AM. The spectra of Cal-590 is well separated from those of FITC, Alexa Fluor 488 and GFP, making it an ideal calcium probe for multiplexing intracellular assays with GFP cell lines or FITC/Alexa Fluor 488 labeled antibodies.
Bioassay Systems QuantiChrom™ Boron Assay Kit. Quantitative determination of boron in agricultural and environmental samples.
For quantitative determination of boron in agricultural and environmental samples. Key Features: Fast and sensitive. Linear detection range: 0.05 to 10 µg/mL (0.05 - 10 ppm) boron with 100 µL sample (96-well). Convenient. The procedure involves adding a single working reagent. High-throughput. "Add-mix-read" type assay. Can be readily automated as a high-throughput 96-well or 384-well plate assay for thousands of samples per day. Method: OD420nm. Samples: Water, plant tisse, soil samples, and antibody conjugation solutions. Species: All. Procedure: Assay takes 40 min. Kit size: 100 tests. Detection limit: 0.05 µg/mL or 0.05 ppm.
Cayman Chemical 2 3cyclIc NADP sodIum sa 7.3mg
A substrate for CNP; has been used in coupled enzyme assays to quantify CNP activity; increases calcium overload-induced calcium release and prevents calcium-induced swelling in rat brain mitochondria at 5 µM
Eurofins Discoverx PathHunter eXpress GPR182 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0754E2CP1M)
The PathHunter GPR182 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR182 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR182 and EA-tagged β-Arrestin-2. Activation of GPR182 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Abcam Cathepsin B Activity Assay Kit (Fluorometric)
Cathepsin B Activity Assay Kit (Fluorometric)
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter eXpress Flt4 Activity Assay, 10-Plate Kit. (93-0888E3CP16L)
The PathHunter FLT4 Activity eXpress kit provides an easy-to-use cell based assay to study small molecule drug candidates targeting RTKs. This kit assesses RTK activity via detection of resulting FLT4 phosphorylation-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged FLT4 to the extracellular ligand binding domain of EGFR alongside an EA-tagged SH2 domain protein. Upon ligand induced receptor activation, cross-phosphorylation of the receptor leads to SH2-EA recruitment and production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Aat Bioquest Cell Meter Autophagy Assay Kit Blue Fluorescence (200 Tests)
Autophagy is an evolutionarily conserved degradation process that targets long-lived proteins organelles and other cytoplasmic components for degradation via the lysosomal pathway. This catalog is for 200 Tests.
Proteintech Group Inc Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells (Calcein AM, EthD-1 Method)
Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells (Calcein AM, EthD-I method) provides dual fluorescence staining for the detection of living and dead cells. The two probes in the kit eflect cell viability by measuring intracellular esterase activity and plasma membrane integrity. This kit can be used for fluorescence microscopes, flow cytometers, microplate readers and other fluorescence detection systems. This kit can be used for most eukaryotic mammalian samples, but not to fungi and yeast.
Amsbio LLC Cupric Iron Chelating (CIC) Assay Kit -store kit components as indicated
Cupric Iron Chelating (CIC) Assay Kit -store kit components as indicated
Revvity Health Sciences Inc HTRF Human & Mouse Phospho-GSK3α (Ser21) Detection Kit, 500 Assay Points
HTRF Human & Mouse Phospho-GSK3α (Ser21) Detection Kit, 500 Assay Points
Indigo Biosciences Human Adrenoceptor Alpha 1B (ADRA1B) All-inclusive Cell-Based Luciferase Reporter Assay Kit, 96-well assay plate format
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO’s Human Adrenoceptor Alpha 1B reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against ADRA1B. Kit includes cryopreserved reporter cells engineered to provide constitutive expression of the receptor, optimized media for use during cell thawing and in diluting test samples, reference agonist, Luciferase Detection Reagent, cell culture-ready assay plate, and detailed assay protocol. INDIGO’s unique Cryomite™ process provides exceptional cell viability post-thaw and INDIGO’s strict QA process means consistent results lot to lot (product quality data available upon request). This kit allows researchers to achieve superior sensitivity, clear, reproducible results, and eliminate weeks of cell culture work.