General Cell Based Assays
Meso Scale Discovery R-PLEX Human MET soluble ASY
The R-PLEX Human Met Soluble Assay from Meso Scale Discovery is a multiplex immunoassay that measures 10 metabolic biomarkers in human serum plasma and cell culture supernatant It uses electrochemiluminescence detection for high sensitivity and broad dynamic range with low sample volume The panel includes insulin leptin PAI-1 total resistin MCP-1 adiponectin TNF-alpha IL-6 CRP and visfatin The assay provides efficient analysis of metabolic and inflammatory status with a protocol time of just over 3 hours It includes a 96-well plate with capture antibodies detection antibodies and calibrators For research use only
Meso Scale Discovery RPLEX NHP Alphaamylase 2A ASY
The R-Plex NHP Alpha-Amylase 2A Assay Kit from Meso Scale Discovery is a 96-well electrochemiluminescent immunoassay for the quantitative measurement of alpha-amylase 2A in human serum plasma saliva and cell culture supernatants The assay uses MSD GOLD SULFO-TAG labels and has a typical sensitivity of 0 04 ng/mL with an assay range of 0 1-400 ng/mL The kit includes a 96-well MULTI-ARRAY Human Alpha-Amylase 2A Plate SULFO-TAG Anti-Alpha-Amylase 2A Antibody Sample Diluent Read Buffer T (4X) and validations packet This assay allows for high-throughput sensitive and accurate quantification of alpha-amylase 2A levels
Meso Scale Discovery R-PLEX Human C1q receptor ASY
The Meso Scale Discovery R-PLEX Human C1q Receptor Assay is a multiplex immunoassay panel that measures C1q Receptor in human serum plasma and cell culture supernatants This assay uses electrochemiluminescence (ECL) detection technology and provides high sensitivity up to 5-logs of dynamic range and low sample volume requirements The assay is available as a singleplex or as a multiplex combining up to 10 biomarkers per well It requires 50 L of sample and can perform up to 80 tests across two plates The assay takes 3 5 hours to complete and has a typical sensitivity of 19 pg/mL
Eurofins Discoverx cAMP Hunter eXpress HRH2 CHO-K1 GPCR Assay, 10-Plate Kit. (95-0043E2CP2L)
The cAMP Hunter HRH2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. histamine) based activation of HRH2 GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type HRH2, a naturally Gs coupled GPCR. Upon activation of HRH2, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery R-PLEX Human AFP ASY
The R-PLEX Human AFP Assay from Meso Scale Diagnostics is an electrochemiluminescent immunoassay for the quantitative measurement of alpha-fetoprotein (AFP) in human serum plasma and tissue culture supernatant This assay utilizes MSD s proprietary SULFO-TAG labels and multi-array technology to provide a highly sensitive and reproducible method for detecting AFP an important biomarker used to help diagnose and monitor hepatocellular carcinoma and other malignancies as well as fetal defects The assay has a wide dynamic range of 1 95-2000 pg/mL and requires only 50 L of sample
Meso Scale Discovery U-PLEX Human IL-17A/F ASY
The Meso Scale Discovery U-PLEX Human IL-17A/F Assay Kit is a multiplex immunoassay that measures human interleukin 17A and interleukin 17F in cell culture supernatants serum plasma and other biological samples This electrochemiluminescent assay utilizes MSD s proprietary SECTOR Imager and provides high sensitivity a broad dynamic range and requires low sample volume The kit includes a 96-well plate coated with capture antibodies detection antibodies Read Buffer Diluent 2 Calibrator Blend and Wash Buffer It can detect IL-17A in a range of 0 06-1500 pg/mL and IL-17F in a range of 0 10-1500 pg/mL
LifeSensors E3LITE Ubiquitin Ligase PLATE only 96-well
The E3LITE Customizable Ubiquitin Ligase Plate consists of a plate that is pre-coated with a proprietary polyubiquitin capture reagent. The resulting product of the E3 ligase-mediated in vitro ubiquitin reaction is measured and represents a direct measure of the E3 ligase activity. The E3LITE Customizable Ubiquitin Plate represents a singular platform for the focused investigation of any E2/E3 enzyme pair, as well as many polyubiquitin linkage types. This assay has been validated for high throughput screening (HTS) and has been employed in the discovery of novel compounds that selectively modulate E3 ligase function. This product is protected by one or more US or Foreign patents.
Meso Scale Discovery R-PLEX AB42 4G8 ASY
The Meso Scale Discovery R-PLEX Human Abeta40 6E10 Assay is a multiplex immunoassay kit for detecting amyloid beta 40 (Abeta40) and amyloid beta bound by antibody 6E10 in human samples like serum plasma and CSF It uses electrochemiluminescence (ECL) technology for high sensitivity and a broad dynamic range The assay requires 50 L or less of sample and can be completed in 3 5 hours It includes a 96-well plate with capture antibodies detection antibodies standards calibrators controls diluent read buffer wash buffer and plate seals
Meso Scale Discovery U-PLEX Mouse TNF-RI ASY
The Meso Scale Discovery U-PLEX Mouse TNF RI Assay Kit is a multiplex immunoassay that measures mouse tumor necrosis factor receptor I (TNF RI) in serum plasma and cell culture supernatants It uses electrochemiluminescence (ECL) detection technology and provides high sensitivity up to 5 logs of dynamic range and low sample volume requirements This assay kit includes a 96-well plate coated with capture antibodies detection antibodies labeled with an ECL tag calibrators and buffer for sample dilution It can test up to 80 samples in duplicate and has a testing time of 3 5 hours The assay has high specificity and shows minimal cross-reactivity
Molecular Devices Assay Kit, Two-Color Fluorescence, Mammalian Cells, 96-Well
The EarlyTox Live/Dead Assay Kit is a two-color fluorescence assay for simultaneous detection of live and dead mammalian cells based on membrane integrity. Designed for fluorescence microplate readers, the Explorer kit is configured for 96-well workflows.
- provides two-color fluorescence detection of live and dead cells
- includes calcein AM and ethidium homodimer-III reagents
- explorer kit size sufficient for two 96-well microplates
- recommended plate: black-walled, clear-bottom 96-well microplates
- excitation/emission: calcein AM 495/530 nm, ethidium homodimer-III 530/645 nm
- store sealed, desiccated, protected from light at -20°C
- use aqueous working solutions within one day and test dye concentrations for optimal results
Revvity Health Sciences Inc HTRF Human MMP1 Detection Kit, 500 Assay Points
HTRF Human MMP1 Detection Kit, 500 Assay Points
Eurofins Discoverx PathHunter eXpress LGR4 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0516E2ACP1L)
The PathHunter LGR4 (GPR48) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of LGR4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged LGR4 and EA-tagged β-Arrestin-2. Activation of LGR4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Revvity Health Sciences Inc HTRF Streptavidin XL665-Conjugate, 5,000 Assay Points
HTRF Streptavidin XL665-Conjugate, 5,000 Assay Points
